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Source Escherichia coli
Amino Acid Sequence
Sequence MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSELDMEEKRRRARVQ GAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQ VVRRAPEPRVIDREGVYEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQ DVPWKQRTGIREDITYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTL KNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGA TRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPK EYHSREPRVNLTFRTVYPDPRGAPW
Molecular weight 38 kDa including tags
Amino acids 1 to 286
Tags His tag N-Terminus
DDDDK tag N-Terminus Specifications
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity > 95
% SDS-PAGE. ab105620 was purified using conventional chromatography techniques.
Concentration information loading... Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0
Function Dioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
Tissue specificity Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
Sequence similarities Belongs to the alkB family. Contains 1 Fe2OG dioxygenase domain.
Cellular localization Cytoplasm. Nucleus.
Information by UniProt
Recombinant Human ALKBH3 protein images
References for Recombinant Human ALKBH3 protein (ab105620)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"