Recombinant Human ALKBH3 protein (ab124851)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ96Q83
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELDM EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW
    • Molecular weight38 kDa including tags
    • Amino acids1 to 286
    • TagsHis tag N-Terminus
    • Additional sequence informationALKBH3 Human Recombinant fused to 39 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 325 amino acids (1-286 a.a.).

Specifications

Our Abpromise guarantee covers the use of ab124851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab124851 is purified by proprietary chromatographic techniques and sterile filtered.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.03% DTT, 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names
    • 1700108H04Rik
    • 1810020C19Rik
    • ABH3
    • AlkB homolog 3
    • alkB, alkylation repair homolog 3 (E. coli)
    • ALKB3_HUMAN
    • ALKBH3
    • Alkylated DNA repair protein alkB homolog 3
    • alkylation repair homolog 3
    • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
    • DEPC 1
    • DEPC-1
    • DEPC1
    • EC 1.14.11.
    • FLJ43614
    • mABH3
    • MGC118790
    • MGC118792
    • MGC118793
    • MGC134125
    • PCA1
    • Prostate cancer antigen 1
    • Prostate cancer antigen-1
    • RP23-375N21.4
    see all
  • FunctionDioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • Tissue specificityUbiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
  • Sequence similaritiesBelongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt

References for Recombinant Human ALKBH3 protein (ab124851)

ab124851 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"