Recombinant Human Aminoacylase 1 protein (ab99247)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMTSKGPEEEHPSVTLFRQYLRIRTVQPKPD YGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNPTLSSILLNSH TDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQYLEAVRRLKV EGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANP TDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFR EKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPD VDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRV CKDMNLTLEPEIMPAATDNRYIRAVGVPALGFSPMNRTPVLLHDHDERLH EAVFLRGVDIYTRLLPALASVPALPSDS
    • Molecular weight
      48 kDa including tags
    • Amino acids
      1 to 408
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab99247 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • ACY 1
    • ACY-1
    • Acy1
    • ACY1_HUMAN
    • ACY1D
    • ACYLASE
    • Acylase I
    • Aminoacylase-1
    • EC 3.5.1.14
    • epididymis secretory protein Li 5
    • HEL-S-5
    • N acyl L amino acid amidohydrolase
    • N-acyl-L-amino-acid amidohydrolase
    • OTTHUMP00000212459
    • OTTHUMP00000212462
    • OTTHUMP00000212463
    • OTTHUMP00000212464
    • OTTHUMP00000212465
    see all
  • Function
    Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).
  • Tissue specificity
    Expression is highest in kidney, strong in brain and weaker in placenta and spleen.
  • Involvement in disease
    Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D) [MIM:609924]. ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids.
  • Sequence similarities
    Belongs to the peptidase M20A family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab99247 (3µg).

References

ab99247 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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