Recombinant human AMPK alpha 2 + AMPK beta 1 +AMPK gamma 3 protein (ab184881)

Overview

  • Product name
    Recombinant human AMPK alpha 2 + AMPK beta 1 +AMPK gamma 3 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Tags
      His tag C-Terminus , proprietary tag N-Terminus
    • Additional sequence information
      Recombinant full-length human AMPK alpha 2, beta 1 and gamma 3 subunits: Swissprot: P54646, Q9Y478 and Q9UGI9. Predicted molecular weights are 92, 65 and 108 kDa. (NM_006252 , NM_006253 , NM_017431).

Specifications

Our Abpromise guarantee covers the use of ab184881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab184881 was determined to be 360 nmol/min/mg.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >90% by SDS-PAGE.
    Assessed by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 5' AMP activated protein kinase beta 1 subunit
    • 5' AMP activated protein kinase subunit beta 1
    • 5' AMP activated protein kinase subunit gamma 3
    • 5' AMP activated protein kinase, catalytic alpha 2 chain
    • 5' AMP activated protein kinase, gamma 3 subunit
    • 5'-AMP-activated protein kinase catalytic subunit alpha-2
    • ACACA kinase
    • Acetyl CoA carboxylase kinase
    • AMP activated protein kinase alpha 2 subunit variant 2
    • AMP activated protein kinase alpha 2 subunit variant 3
    • AMP activated protein kinase beta subunit
    • AMPK
    • AMPK alpha 2 chain
    • AMPK beta 1 chain
    • AMPK beta 1
    • AMPK gamma 3 chain
    • AMPK gamma3
    • AMPK subunit alpha 2
    • AMPK subunit beta 1
    • AMPK subunit gamma 3
    • AMPK2
    • AMPKa2
    • AMPKb
    • AMPKG3
    • HAMPKb
    • HMGCR kinase
    • Hydroxymethylglutaryl CoA reductase kinase
    • PRKAA
    • Protein kinase, AMP activated, gamma 3 non catalytic subunit
    • Protein kinase, AMP activated, noncatalytic, beta 1
    • Protein kinase, AMP-activated, alpha 2 catalytic subunit
    • Protein kinase, AMP-activated, beta 1 non-catalytic subunit
    see all
  • Relevance
    The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
  • Cellular localization
    Cytoplasmic and Nuclear

Images

  • The specific activity of ab184881 was determined to be 360 nmol/min/mg.

  • ab184881 was determined to be >90% pure by densitometry.

References

ab184881 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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