The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Binds STAM protein with Kd = ~7 µM. In in vitro assays containing 200 nM AMSH, a ~10-fold increase in the rate of hydrolysis of K63-linked diubiquitin FRET substrate was achieved upon the addition of STAM. Half-maximal increase occurred at 0.4 µM STAM.
Typical enzyme concentration for use in vitro ranges from 100-500 nM depending on conditions and substrate. Suggested Reaction Buffer for plate-based assays is 50 mM Hepes, pH 8.0, 100 mM NaCl, and 0.1 mg/ml BSA. AMSH activity is partially or fully inhibited by DTT at concentrations above 1 mM.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Associated molecule with the SH3 domain of STAM
Endosome associated ubiquitin isopeptidase
Endosome-associated ubiquitin isopeptidase
STAM binding protein
Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
Belongs to the peptidase M67C family. Contains 1 MPN (JAB/Mov34) domain.
The JAMM motif is essential for the protease activity.
Phosphorylated after BMP type I receptor activation. Ubiquitinated by SMURF2 in the presence of RNF11.