Recombinant human Angiopoietin 1 protein (ab69492)

Overview

Description

  • NatureRecombinant
  • SourceHeLa cells
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceSequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein.

Specifications

Our Abpromise guarantee covers the use of ab69492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.
    Greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 5% Trehalose, 200mM Sodium chloride, 20mM Sodium phosphate, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitution should be done in water to a concentration of 0.1-1.0mg/ml.

General Info

  • Alternative names
    • AGP 1
    • AGP1
    • AGPT
    • ANG
    • ANG 1
    • ANG-1
    • ANG1
    • Angiopoietin-1
    • Angiopoietin1
    • ANGP1_HUMAN
    • ANGPT 1
    • Angpt1
    • KIAA0003
    • OTTHUMP00000227592
    • OTTHUMP00000227599
    • OTTHUMP00000227600
    see all
  • FunctionBinds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human Angiopoietin 1 protein (ab69492)

ab69492 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I have attached the results of the sequence alignment between mouse and human angiopoietin 1.

The link to our IHC-P protocol is as follows:
http://www.abcam.com/index.html?pageconfig=resource&r...

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Thank you for your inquiry. I can confirm that ab69492 is an active human Angiopoietin 1 protein that showed biological activity determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC)...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"