Recombinant human Angiopoietin 1 protein (ab69492)
Key features and details
- Expression system: HeLa cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Angiopoietin 1 protein
See all Angiopoietin 1 proteins and peptides -
Biological activity
Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC).
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Purity
> 95 % SDS-PAGE.
Greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
Expression system
HeLa cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein. -
Tags
His tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab69492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 5% Trehalose, 0.328% Sodium phosphate, 1.16% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitution should be done in water to a concentration of 0.1-1.0mg/ml.
General Info
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Alternative names
- AGP 1
- AGP1
- AGPT
see all -
Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab69492 has been referenced in 1 publication.
- Hultström M et al. Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients. Biomedicines 10:N/A (2022). PubMed: 35740360