Recombinant human Angiopoietin 2 protein (ab123226)

Overview

Description

  • NatureRecombinant
  • SourceCHO cells
  • Amino Acid Sequence
    • AccessionO15123
    • SpeciesHuman
    • SequenceDAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQQNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE LQLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHIIQLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QHDLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTNGI YT
    • Amino acids68 to 299
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityDetermined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2µg/ml.
  • Applications

    SDS-PAGE

    HPLC

    Functional Studies

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.
    Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
  • FormLyophilised
  • Additional notes Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2µg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionWe recommend storing the lyophilised product at -20ºC. If the product has been reconstituted, then we recommend storing with a carrier protein, such as 0.1% BSA in working aliquots at -20ºC to -80ºC for 3 months.

General Info

  • Alternative names
    • AGPT 2
    • Agpt2
    • ANG 2
    • ANG-2
    • ANG2
    • Angiopoietin 2a
    • Angiopoietin 2B
    • Angiopoietin-2
    • Angiopoietin2
    • ANGP2_HUMAN
    • ANGPT 2
    • Angpt2
    • Tie2 ligand
    see all
  • FunctionCan induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainThe Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human Angiopoietin 2 protein (ab123226)

ab123226 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"