The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityDetermined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2µg/ml.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2µg/ml.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionWe recommend storing the lyophilised product at -20ºC. If the product has been reconstituted, then we recommend storing with a carrier protein, such as 0.1% BSA in working aliquots at -20ºC to -80ºC for 3 months.
FunctionCan induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.