The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivates ERK phosphorylation in THP-1 cells.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. ab108558 is 0.2 µm filtered.
Additional notesWorking aliquots are stable for up to 3 months when stored at -20°C.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Angiopoietin like 4
Angiopoietin related protein 4
Angiopoietin-like protein 4
Angiopoietin-related protein 4
Fasting induced adipose factor
Hepatic angiopoietin related protein
Hepatic fibrinogen/angiopoietin related protein
Hepatic fibrinogen/angiopoietin-related protein
Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein
PPARG angiopoietin related protein
Weakly similar to angiopoietin 1 [H.sapiens]
FunctionProtein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
Tissue specificityExpressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
Cellular localizationSecreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4.
Recombinant human Angiopoietin-like 4 protein images
Functional Studies - ARP4 protein (ab108558)
ERK phosphorylation induced by ab108558 (hANGPTL4 - coiled-coil domain) in THP-1 cells.
A. THP-1 monocyte cells were serum starved for 16 hr and then stimulated with ab108558 (500 ng/ml) for 1, 2 and 3 hrs, respectively. Antibodies against pERK1/2
and total ERK1/2 were used for immunoblotting.
B. Cells were treated with 20 µM PD98058 (MEK inhibitor) for 30 min prior adding of 500 ng/ml ab108558. After 3 hrs of incubation, Western blot was performed.
References for Recombinant human Angiopoietin-like 4 protein (ab108558)
has not yet been referenced specifically in any publications.
Publishing research using ab108558? Please let us know so that we can cite the reference in this datasheet.
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