Recombinant human Angiostatin protein (ab123771)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP00747
    • SpeciesHuman
    • SequenceVYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLKGTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGKRAPWCHT TNSQVRWEYC KIPSCDSSP
    • Molecular weight30 kDa
    • Amino acids98 to 259
    • Additional sequence informationab123771 is a single, non-glycosylated polypeptide chain, it represents a proteolytic fragment of plasminogen containing the first three kringle structures.

Specifications

Our Abpromise guarantee covers the use of ab123771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 0.55×105 Units/mg.
  • Applications

    Functional Studies

    HPLC

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purity is greater than 95% as determined by HPLC and SDS-PAGE.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Store under Desiccating conditions.

    pH: 5.50
    Constituents: 4% Mannitol, 0.16% Sodium acetate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, subdivide the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

General Info

  • RelevanceAngiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains. The Kringle domains have specific anti-migratory and anti-proliferative activities. Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.
  • Cellular localizationSecreted

References for Recombinant human Angiostatin protein (ab123771)

ab123771 has not yet been referenced specifically in any publications.

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