Recombinant Human Annexin A2 protein (ab93005)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab93005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

    Western blot

    ELISA

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 100mM Imidazole
    Constituents: 0.5M Sodium chloride, 20mM Tris HCl, 1mM Beta mercaptoethanol, pH 8.0

General Info

  • Alternative names
    • Annexin A2
    • Annexin II
    • Annexin II, heavy chain
    • Annexin-2
    • ANX 2
    • ANX2
    • ANX2L4
    • ANXA2
    • ANXA2_HUMAN
    • arylsulfatase B
    • CAL1H
    • Calpactin I heavy chain
    • Calpactin I heavy polypeptide
    • calpactin I heavy polypeptide (p36)
    • Calpactin-1 heavy chain
    • chromobindin 8
    • Chromobindin-8
    • Epididymis secretory protein Li 270
    • HEL S 270
    • LIP2
    • Lipocortin II
    • LPC2
    • LPC2D
    • p36
    • P36 protein
    • PAP-IV
    • Placental anticoagulant protein IV
    • Protein I
    see all
  • FunctionCalcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt

Recombinant Human Annexin A2 protein images

  • ab93005 analysed by 10% SDS-PAGE stained with Coomassie Blue (CB) and immunoblotted with anti-6x His serum (WB).

References for Recombinant Human Annexin A2 protein (ab93005)

ab93005 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ELISA
We coated 0.2 ug of this protein in carbonate-bicarbonate buffer (pH9.5) into each well on 96-well plate (high binding, Corning) for ELISA assay, the coated annexin a2 (ANXA2) worked very well for ELISA assay.
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Submitted Nov 05 2015

Abreviews
Application Functional Studies
We co-incubated this protein and its interacting partner for 2 hours at RT followed by native band shift electrophoresis and immunological blot assays, the result showed that this ANXA2 protein is extremely stable and functional for the assay.
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Submitted Nov 02 2015

Thank you for contacting us. Unfortunately, the Annexin II proteins ab93005 and ab81777 were not tested for their biological activity. Also, as the purification process may influence the conformation of the protein, we cannot guarantee t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"