Recombinant Human Annexin VI protein (ab92968)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      1 to 673

Specifications

Our Abpromise guarantee covers the use of ab92968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

    Other

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 100mM Imidazole
    Constituents: 0.5M Sodium chloride, 20mM Tris HCl, 1mM Beta mercaptoethanol, pH 8

General Info

  • Alternative names
    • 67 kDa calelectrin
    • Annexin A6
    • Annexin VI
    • Annexin VI p68
    • Annexin-6
    • AnnexinA6
    • AnnexinVI
    • ANX 6
    • ANX A6
    • ANX6
    • ANXA 6
    • ANXA6
    • ANXA6_HUMAN
    • Calcium binding protein p68
    • Calelectrin
    • Calphobindin II
    • Calphobindin-II
    • CalphobindinII
    • CBP 68
    • CBP68
    • Chromobindin 20
    • Chromobindin-20
    • Chromobindin20
    • CPB II
    • CPB-II
    • CPBII
    • Lipocortin VI
    • LipocortinVI
    • p68
    • p70
    • Protein III
    • ProteinIII
    see all
  • Function
    May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 8 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylated in response to growth factor stimulation.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • 10% SDS-PAGE showing ab92968
    Lane 1 MWM
    Lane 2 Stained with Coomassie Blue
    Lane 3 Immunobloting with anti-6xHis serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry
  • Anti-Annexin VI antibody (ab52221) at 1/1000 dilution + Recombinant Human Annexin VI protein (ab92968) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References

ab92968 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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