Recombinant Human APG5L/ATG5 protein (ab40469)



  • Nature
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species

Associated products


Our Abpromise guarantee covers the use of ab40469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry


  • Purity
    > 95 % SDS-PAGE.

  • Form
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: 0.002% Sodium Azide
    Constituents: 0.1% Triton-X-100, 10mM Tris, 10mM DTT, pH 8.0

General Info

  • Alternative names
    • APG 5
    • APG 5L
    • APG5
    • APG5 autophagy 5 like
    • APG5 like
    • APG5-like
    • APG5L
    • Apoptosis specific protein
    • Apoptosis-specific protein
    • ASP
    • ATG 5
    • Atg5
    • ATG5 autophagy related 5 homolog
    • ATG5_HUMAN
    • Autophagy protein 5
    • Autophagy related 5
    • hAPG5
    • Homolog of S Cerevisiae autophagy 5
    • OTTHUMP00000040507
    see all
  • Function
    Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
    May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
  • Tissue specificity
    Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
  • Sequence similarities
    Belongs to the ATG5 family.
  • Post-translational
    Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
    Acetylated by EP300.
  • Cellular localization
    Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
  • Information by UniProt

Recombinant Human APG5L/ATG5 protein images

  • Analysis of APG5L Recombinant Protein.
    4-20% SDS gradient gel. Coomassie blue staining.

References for Recombinant Human APG5L/ATG5 protein (ab40469)

ab40469 has not yet been referenced specifically in any publications.

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