Recombinant Human Apolipoprotein A V (ab113861)

Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionQ6Q788
    • SpeciesHuman
    • SequenceRKGFWDYFSQ TSGDKGRVEQ IHQQKMAREP ATLKDSLEQD LNNMNKFLEK LRPLSGSEAP RLPQDPVGMR RQLQEELEEV KARLQPYMAE AHELVGWNLE GLRQQLKPYT MDLMEQVALR VQELQEQLRV VGEDTKAQLL GGVDEAWALL QGLQSRVVHH TGRFKELFHP YAESLVSGIG RHVQELHRSV APHAPASPAR LSRCVQVLSR KLTLKAKALH ARIQQNLDQL REELSRAFAG TGTEEGAGPD PQMLSEEVRQ RLQAFRQDTY LQIAAFTRAI DQETEEVQQQ LAPPPPGHSA FAPEFQQTDS GKVLSKLQAR LDDLWEDITH SLHDQGHSHL GDPAAADYKD DDDK
    • Molecular weight40 kDa including tags
    • Amino acids24 to 366
    • TagsDDDDK tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab113861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    ELISA

  • Endotoxin level< 1.000 Eu/µg
  • Purity> 70 % SDS-PAGE.
    Purity determined by densitometric image analysis
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.24% Tris, 0.29% Sodium chloride

  • ReconstitutionAdd deionized water (200 µl) to prepare a working stock solution of 0.5 mg/mL

General Info

  • Alternative names
    • Apo-AV
    • ApoA-V
    • Apoa5
    • APOA5_HUMAN
    • ApoAV
    • Apolipoprotein A-V
    • Apolipoprotein A5
    • RAP3
    • Regeneration associated protein 3
    • Regeneration-associated protein 3
    see all
  • FunctionMinor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages.
  • Tissue specificityLiver and plasma.
  • Involvement in diseaseDefects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR)[MIM:145750].mFamilial hypertriglyceridemia is a common inherited disorder in which the concentration of very low density lipoprotein (VLDL) is elevated in the plasma. This leads to increased risk of heart disease, obesity, and pancreatitis.
    Defects in APOA5 are a cause of hyperlipoproteinemia type 5 (HLPP5) [MIM:144650]. HLPP5 is characterized by increased amounts of chylomicrons and very low density lipoprotein (VLDL) and decreased low density lipoprotein (LDL) and high density lipoprotein (HDL) in the plasma after a fast. Numerous conditions cause this phenotype, including insulin-dependent diabetes mellitus, contraceptive steroids, alcohol abuse, and glycogen storage disease type 1A (GSD1A) [MIM:232200].
  • Sequence similaritiesBelongs to the apolipoprotein A1/A4/E family.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant Human Apolipoprotein A V images

  • 12% SDS-PAGE separation of Human Apolipoprotein A V
    1.reduced and boiled sample, 5µg/lane
    2.non-reduced and non-boiled sample, 5µg/lane

References for Recombinant Human Apolipoprotein A V (ab113861)

ab113861 has not yet been referenced specifically in any publications.

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