Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      649 to 946

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Form
    Liquid
  • Additional notes

    ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Apoptosis signal regulating kinase 1
    • Apoptosis signal-regulating kinase 1
    • ASK 1
    • ASK-1
    • ASK1
    • M3K5
    • M3K5_HUMAN
    • MAP/ERK kinase kinase 5
    • MAP3K5
    • MAPK/ERK kinase kinase 5
    • MAPKKK5
    • MEK kinase 5
    • MEKK 5
    • MEKK5
    • Mitogen activated protein kinase kinase kinase 5
    • Mitogen-activated protein kinase kinase kinase 5
    see all
  • Function
    Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
  • Tissue specificity
    Abundantly expressed in heart and pancreas.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dephosphorylated and activated by PGAM5.
  • Information by UniProt

Images

  • Sample Kinase Activity Plot.
  • ab60325 on SDS-PAGE, MW ~60kDa.

References

ab60325 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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