The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
APG4 autophagy 4 homolog A
ATG4 autophagy related 4 homolog A
ATG4 autophagy related 4 homolog A (S. cerevisiae)
AUT like 2 cysteine endopeptidase
AUT-like 2 cysteine endopeptidase
Autophagy related 4A cysteine peptidase
Autophagy related cysteine endopeptidase 2
Autophagy related protein 4 homolog A
Autophagy-related cysteine endopeptidase 2
Autophagy-related protein 4 homolog A
Cysteine protease ATG4A
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.