Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE LVEQQPSHLA CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSLLEHHHHH H
    • Molecular weight
      45 kDa including tags
    • Amino acids
      1 to 393
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab123195 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.002% PMSF, 0.02% DTT, 0.32% Tris HCl, 20% Glycerol

General Info

  • Alternative names
    • APG4 autophagy 4 homolog B
    • APG4B
    • ATG4 autophagy related 4 homolog B (S. cerevisiae)
    • ATG4B
    • ATG4B_HUMAN
    • AUT like 1 cysteine endopeptidase
    • AUT-like 1 cysteine endopeptidase
    • AUTL1
    • Autophagin 1
    • Autophagin-1
    • Autophagy related 4B cysteine peptidase
    • Autophagy related cysteine endopeptidase 1
    • Autophagy related protein 4 homolog B
    • Autophagy-related cysteine endopeptidase 1
    • Autophagy-related protein 4 homolog B
    • Cysteine protease ATG4B
    • hAPG4B
    • MGC1353
    see all
  • Function
    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificity
    Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • Sequence similarities
    Belongs to the peptidase C54 family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab123195 (3µg).

References

ab123195 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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