Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9Y4P1
    • SpeciesHuman
    • SequenceMDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE LVEQQPSHLA CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSLLEHHHHH H
    • Molecular weight45 kDa including tags
    • Amino acids1 to 393
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab123195 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.002% PMSF, 0.02% DTT, 0.32% Tris HCl, 20% Glycerol

General Info

  • Alternative names
    • APG4 autophagy 4 homolog B
    • APG4B
    • ATG4 autophagy related 4 homolog B (S. cerevisiae)
    • ATG4B
    • ATG4B_HUMAN
    • AUT like 1 cysteine endopeptidase
    • AUT-like 1 cysteine endopeptidase
    • AUTL1
    • Autophagin 1
    • Autophagin-1
    • Autophagy related cysteine endopeptidase 1
    • Autophagy related protein 4 homolog B
    • Autophagy-related cysteine endopeptidase 1
    • Autophagy-related protein 4 homolog B
    • Cysteine protease ATG4B
    • hAPG4B
    • MGC1353
    see all
  • FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificityMainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • Sequence similaritiesBelongs to the peptidase C54 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant Human ATG4B protein images

  • 15% SDS-PAGE showing ab123195 (3µg).

References for Recombinant Human ATG4B protein (ab123195)

ab123195 has not yet been referenced specifically in any publications.

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