The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
APG4 autophagy 4 homolog D
APG4, S. cerevisiae, homolog of, D
ATG4 autophagy related 4 homolog D (S. cerevisiae)
AUT like 4 cysteine endopeptidase
AUT-like 4 cysteine endopeptidase
autophagy 4, S. cerevisiae, homolog of, D
autophagy related 4D, cysteine peptidase
Autophagy related cysteine endopeptidase 4
Autophagy related protein 4 homolog D
Autophagy-related cysteine endopeptidase 4
Autophagy-related protein 4 homolog D
Cysteine protease ATG4D
Cysteine protease involved in autophagy
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Mainly expressed in skeletal muscle and, to a lower extent, in testis.