Recombinant human BRAF + Cdc37 protein (ab42596)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 25% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: Inhibition Assay
Description
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Product name
Recombinant human BRAF + Cdc37 protein -
Biological activity
Specific Activity: 12 pmol/min/μg.
Activity was tested using 1 μM MEK1 (K97R) as substrate. Assay was performed in a 50 μl reation in 10 mM Tris, pH 7.4, 5 mM
MgCl2 , 0.5 mM EDTA, 0.5 mM DTT, 5 μM ATP, 5 μg MEK1 (K97R) as substrate, and B Raf. 62 ng of B Raf enzyme phosphorylated over 18% of 1 μM MEK1 at room temperature in 1 hr.
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Purity
> 25 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS LPKIH -
Tags
His tag N-Terminus , DDDDK tag N-Terminus -
Additional sequence information
Human BRAF (NM_004333, a.a.432-725, N-term His-tag, 36 kDa), and p50 (NM_007065, a.a.2-378 (end), N-term FLAG-tag, 45 kDa). Protein exists in a complex with Cdc37 and p50.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab42596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Inhibition Assay
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Form
Liquid -
Additional notes
Coexpressed in Baculovirus infected Sf9 cell expression system.
Used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.53% Imidazole
Constituents: 0.018% Potassium chloride, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.7% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.72% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Cellular localization
B Raf: Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. Cdc37: Cytoplasm.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab42596 has not yet been referenced specifically in any publications.