Recombinant human B Raf + Cdc37 protein (ab42596)

Overview

  • Product nameRecombinant human B Raf + Cdc37 protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceSEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS LPKIH
    • TagsHis tag N-Terminus , DDDDK tag N-Terminus
    • Additional sequence informationHuman BRAF (NM_004333, a.a.432-725, N-term His-tag, 36 kDa), and p50 (NM_007065, a.a.2-378 (end), N-term FLAG-tag, 45 kDa). Protein exists in a complex with Cdc37 and p50.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab42596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: 12 pmol/min/μg.

    Activity was tested using 1 μM MEK1 (K97R) as substrate. Assay was performed in a 50 μl reation in 10 mM Tris, pH 7.4, 5 mM

    MgCl2 , 0.5 mM EDTA, 0.5 mM DTT, 5 μM ATP, 5 μg MEK1 (K97R) as substrate, and B Raf. 62 ng of B Raf enzyme phosphorylated over 18% of 1 μM MEK1 at room temperature in 1 hr.

  • Applications

    Inhibition Assay

  • FormLiquid
  • Additional notes

    Coexpressed in Baculovirus infected Sf9 cell expression system.

    Used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.53% Imidazole
    Constituents: 0.018% Potassium chloride, 0.0462% DTT, 0.7% Tris HCl, 10% Glycerol, 0.72% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Cellular localizationB Raf: Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. Cdc37: Cytoplasm.

Recombinant human B Raf + Cdc37 protein images

  • 10% SDS-PAGE with coomassie staining.  

    Left lane: 4 µg B Raf

    Right lane: Protein marker

References for Recombinant human B Raf + Cdc37 protein (ab42596)

ab42596 has not yet been referenced specifically in any publications.

Product Wall

Thanks for your reply, and I'm happy to hear that ab55690 will work as a replacement.

I'm sending a free of charge vial on the order ***, which is on back order but is expected to arrive on Wednesday, March 7.

Please keep me updated...

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Thank you for your reply and for your patience. I forwarded the assay details to the lab to see if there was any explanation for these results.

I have been informedthat there is 250 mM imidazole in the His-BRaf formulation. Imidazoleinterfere...

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Thank you for contactin us and letting us know about this issue with ab42596.

I am sorry to hear about the trouble with this protein, andour files indicatethat you have previously contacted us about a delay in shipping this order to you.
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Thank you for contacting Abcam regarding ab42595.

I am sorry that you have been experiencing difficulties with this protein. I have checked into the shipping error you referred to, and it seems that there was a delay in getting this shipment...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"