The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Measured by its ability to inhibit anti-CD3 antibody induced IL2 secretion in Human T lymphocytes. The ED50 for this effect is typically 0.75 - 3 µg/ml.
< 1.000 Eu/µg
>90% by SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.4 Constituents: 5% Trehalose, 95% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
B7 family member, H4
B7 homolog 4
B7 superfamily member 1
B7 superfamily, member 1
Immune costimulatory protein B7-H4
Immune costimulatory protein B7H4
T cell costimulatory molecule B7x
T-cell costimulatory molecule B7x
V set domain-containing T cell activation inhibitor 1
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.
Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Cell membrane. Expressed at the cell surface. A soluble form has also been detected.