Recombinant Human Band 3 protein (ab114402)

Overview

  • Product nameRecombinant Human Band 3 protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionP02730
    • SpeciesHuman
    • SequencePIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRIDAYMAQSRGELLHS LEGFLDCSLVLPPTDAPSEQALLSLVPVQRELLRRRYQSSPAKPDSSFYK
    • Molecular weight37 kDa including tags
    • Amino acids261 to 360

Specifications

Our Abpromise guarantee covers the use of ab114402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Western blot

  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.03% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • AE 1
    • AE1
    • Anion exchange protein 1
    • Anion exchanger 1
    • B3AT_HUMAN
    • Band 3 anion transport protein
    • BND3
    • CD233
    • DI
    • Diego blood group
    • EMPB3
    • EPB3
    • Erythrocyte membrane protein band 3
    • Erythroid anion exchange protein
    • FR
    • Froese blood group
    • RTA1A
    • SLC4A1
    • Solute carrier family 4 anion exchanger member 1
    • Solute carrier family 4 member 1
    • SW
    • Swann blood group
    • Waldner blood group
    • WD
    • WD1
    • WR
    • Wright blood group
    see all
  • FunctionFunctions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
  • Tissue specificityDetected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
  • Involvement in diseaseOvalocytosis, Southeast Asian
    Spherocytosis 4
    Renal tubular acidosis, distal, autosomal dominant
    Renal tubular acidosis, distal, with hemolytic anemia
    Renal tubular acidosis, distal, with normal red cell morphology
    Cryohydrocytosis
  • Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications
    Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
    Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
    N-glycosylated.
  • Cellular localizationCell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
  • Information by UniProt

Recombinant Human Band 3 protein images

  • 12.5% SDS-PAGE analysis of ab114402, stained with Coomassie Blue.

References for Recombinant Human Band 3 protein (ab114402)

ab114402 has not yet been referenced specifically in any publications.

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