Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      KVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGP TLSRMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLS
    • Molecular weight
      37 kDa including tags
    • Amino acids
      61 to 160

Specifications

Our Abpromise guarantee covers the use of ab152850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 µg/µl.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • BAP 1
    • Bap1
    • BAP1_HUMAN
    • BRCA 1 associated protein 1
    • BRCA1 associated protein 1
    • BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
    • BRCA1-associated protein 1
    • Cerebral protein 13
    • Cerebral protein 6
    • DKFZp686N04275
    • FLJ35406
    • FLJ37180
    • HUCEP 13
    • Hucep 6
    • HUCEP13
    • Hucep6
    • KIAA0272
    • TPDS
    • Ubiquitin carboxy terminal hydrolase
    • Ubiquitin carboxyl terminal hydrolase BAP 1
    • Ubiquitin carboxyl terminal hydrolase BAP1
    • Ubiquitin carboxyl-terminal hydrolase BAP1
    • UCHL2
    see all
  • Function
    Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.
  • Tissue specificity
    Highly expressed in testis, placenta and ovary. Expressed in breast.
  • Sequence similarities
    Belongs to the peptidase C12 family. BAP1 subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
  • Information by UniProt

Images

  • 12.5% SDS-PAGE analysis of ab152850 stained with Coomassie Blue.

References

ab152850 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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