Recombinant Human BCKDHA protein (ab104900)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQCYGNISD LGKGRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RANANRVVIC YFGEGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEEQEKAWRK QSRRKVMEAF EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLARHLQTY GEHYPLDHFD K
    • Molecular weight
      48 kDa including tags
    • Amino acids
      46 to 445
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab104900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 80 % SDS-PAGE.
    ab104900 was purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 5mM DTT, 200mM Sodium chloride, 20mM Tris HCl, pH 8

General Info

  • Alternative names
    • Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide
    • FLJ45695
    • OVD1A
    • 2 oxoisovalerate dehydrogenase (lipoamide)
    • 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial
    • 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
    • BCKDE1A
    • BCKDH E1 alpha
    • BCKDH E1-alpha
    • BCKDHA
    • Branched chain alpha keto acid dehydrogenase E1 component alpha chain
    • Branched chain keto acid dehydrogenase E1 alpha polypeptide
    • Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease)
    • Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
    • MSU
    • MSUD1
    • ODBA_HUMAN
    see all
  • Function
    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease
    Maple syrup urine disease 1A
  • Sequence similarities
    Belongs to the BCKDHA family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3ug ab104900

References

ab104900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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