Recombinant Human beta Tubulin protein (ab70187)


  • Product nameRecombinant Human beta Tubulin protein
  • Protein lengthFull length protein


  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Molecular weight76 kDa including tags
    • Amino acids1 to 444
    • TagsGST tag N-Terminus


Our Abpromise guarantee covers the use of ab70187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot


  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.036% PMSF, 0.089% DTT, 17.819% Tris HCl, 25% Glycerol, 53.457% Sodium chloride, 3.564% Glutathione, 0.036% EDTA

General Info

  • Alternative names
    • Beta 4 tubulin
    • Beta 5 tubulin
    • beta Ib tubulin
    • Beta1 tubulin
    • Class I beta tubulin
    • M40
    • MGC117247
    • MGC16435
    • OK/SW cl.56
    • OK/SWcl.56
    • TBB5_HUMAN
    • TUBB
    • TUBB 1
    • TUBB 2
    • TUBB 5
    • TUBB1
    • TUBB2
    • TUBB5
    • tubulin beta 1 chain
    • Tubulin beta 2 chain
    • tubulin beta 5 chain
    • Tubulin beta chain
    • Tubulin beta class I
    • tubulin beta polypeptide
    • Tubulin beta-5 chain
    see all
  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Involvement in diseaseCortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • Cellular localizationCytoplasm, cytoskeleton.
  • Information by UniProt

Recombinant Human beta Tubulin protein images

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Recombinant Human beta Tubulin protein (ab70187)

ab70187 has not yet been referenced specifically in any publications.

Product Wall

ab70187 contains a N-terminal GST tag, which is 26 kDa.

We also have an extensive range of beta tubulin antibodies if you are looking for one through the link below:

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