Recombinant Human beta Tubulin protein (ab70187)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      76 kDa including tags
    • Amino acids
      1 to 444
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab70187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.036% PMSF, 0.089% DTT, 17.819% Tris HCl, 25% Glycerol, 53.457% Sodium chloride, 3.564% Glutathione, 0.036% EDTA

General Info

  • Alternative names
    • Beta 4 tubulin
    • Beta 5 tubulin
    • beta Ib tubulin
    • Beta1 tubulin
    • Class I beta tubulin
    • M40
    • MGC117247
    • MGC16435
    • OK/SW cl.56
    • OK/SWcl.56
    • TBB5_HUMAN
    • TUBB
    • TUBB 1
    • TUBB 2
    • TUBB 5
    • TUBB1
    • TUBB2
    • TUBB5
    • tubulin beta 1 chain
    • Tubulin beta 2 chain
    • tubulin beta 5 chain
    • Tubulin beta chain
    • Tubulin beta class I
    • tubulin beta polypeptide
    • Tubulin beta-5 chain
    see all
  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificity
    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Involvement in disease
    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • Sequence similarities
    Belongs to the tubulin family.
  • Domain
    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt

Images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References

ab70187 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

ab70187 contains a N-terminal GST tag, which is 26 kDa.



We also have an extensive range of beta tubulin antibodies if you are looking for one through the link below:



http://www.abcam.com/products?keywords=beta+...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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