The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 0.05 ng/ml; corresponding to a Specific Activity of > 2.0 ×107IU/mg.
% SDS-PAGE. ab123846 was purified by proprietary chromatographic techniques. Purity is greater than 97% as determined by HPLC and SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Store under desiccating conditions.
pH: 7.40 Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Centrifuge the vial prior to opening. Reconstitute in sterile distilled H2O to a concentration of 100 µg/ml. This solution can then be diluted into other aqueous buffers. Reconstituted ab123846 should be
stored at 4°C for 2-7 days and at -20°C for future use. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA) and store as working aliquots at -20°C.
Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors.
Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine.