Recombinant human Betacellulin protein (ab123846)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP35070
    • SpeciesHuman
    • SequenceDGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGR CRFVVA EQTPSCVCDEGYIGARCERVDLFY
    • Molecular weight9 kDa
    • Amino acids32 to 111

Specifications

Our Abpromise guarantee covers the use of ab123846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 0.05 ng/ml; corresponding to a Specific Activity of > 2.0 ×107IU/mg.
  • Applications

    HPLC

    Functional Studies

    SDS-PAGE

  • Purity> 97 % SDS-PAGE.
    ab123846 was purified by proprietary chromatographic techniques. Purity is greater than 97% as determined by HPLC and SDS-PAGE.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Store under Desiccating conditions.

    pH: 7.40
    Constituent: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in sterile distilled H2O to a concentration of 100 µg/ml. This solution can then be diluted into other aqueous buffers. Reconstituted ab123846 should be stored at 4°C for 2-7 days and at -20°C for future use. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA) and store as working aliquots at -20°C.

General Info

  • Alternative names
    • Betacellulin
    • Betacellulin precursor
    • BTC
    • BTC_HUMAN
    • OTTHUMP00000160600
    • OTTHUMP00000219057
    • Probetacellulin
    see all
  • FunctionPotent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors.
  • Tissue specificitySynthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine.
  • Sequence similaritiesContains 1 EGF-like domain.
  • Cellular localizationCell membrane and Secreted > extracellular space.
  • Information by UniProt

References for Recombinant human Betacellulin protein (ab123846)

ab123846 has not yet been referenced specifically in any publications.

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