Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ8WV28
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVS
    • Molecular weight53 kDa including tags
    • Amino acids1 to 456
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab104120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, 0.1mM PMSF, pH 8

General Info

  • Alternative names
    • AGM4
    • B cell adapter containing SH2 domain protein
    • B cell adapter containing Src homology 2 domain protein
    • B cell adaptor containing SH2 domain
    • B cell linker
    • B cell linker protein
    • B cell-specific adaptor protein
    • B-cell activation
    • B-cell adapter containing a SH2 domain protein
    • B-cell adapter containing a Src homology 2 domain protein
    • B-cell linker protein
    • BASH
    • Bca
    • BLNK
    • BLNK s
    • BLNK_HUMAN
    • Cytoplasmic adapter protein
    • Ly 57
    • Ly57
    • Lymphocyte antigen 57
    • Lyw 57
    • Lyw57
    • MGC111051
    • SH2 domain-containing leukocyte protein, 65-KD
    • SLP 65
    • SLP-65
    • SLP65
    • Src homology [SH2] domain-containing leukocyte protein of 65 kD
    • Src homology 2 domain containing leukocyte protein of 65 kDa
    • Src homology 2 domain-containing leukocyte protein of 65 kDa
    see all
  • FunctionFunctions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
  • Tissue specificityExpressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
  • Involvement in diseaseDefects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • Sequence similaritiesContains 1 SH2 domain.
  • Post-translational
    modifications
    Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
  • Cellular localizationCytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
  • Information by UniProt

Recombinant Human BLNK protein images

  • 15% SDS-PAGE analysis of ab104120 (3 µg).

References for Recombinant Human BLNK protein (ab104120)

ab104120 has not yet been referenced specifically in any publications.

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