Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP

Specifications

Our Abpromise guarantee covers the use of ab85485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    ab85485 was purified by using conventional chromatography.
  • FormLiquid
  • Additional notesEndotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method )
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 7.5

General Info

  • Alternative names
    • Bcl 2 modifying factor
    • Bcl-2-modifying factor
    • Bcl2 modifying factor
    • Bmf
    • BMF_HUMAN
    • FLJ00065
    see all
  • FunctionMay play a role in apoptosis. Isoform 1 seems to be the main initiator.
  • Tissue specificityIsoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • Information by UniProt

Recombinant Human Bmf protein images

  • Anti-Bmf antibody (ab9655) at 2 µg/ml + Recombinant Human Bmf protein (ab85485) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes
  • 15% SDS-PAGE analysis of 3µg ab85485

References for Recombinant Human Bmf protein (ab85485)

ab85485 has not yet been referenced specifically in any publications.

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