Recombinant Human BMP5 protein (ab87627)

Overview

  • Product nameRecombinant Human BMP5 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMAANKRKNQN RNKSSSHQDS SRMSSVGDYN TSEQKQACKK HELYVSFRDL GWQDWIIAPE GYAAFYCDGE CSFPLNAHMN ATNHAIVQTL VHLMFPDHVP KPCCAPTKLN AISVLYFDDS SNVILKKYRN MVVRSCGCH

Specifications

Our Abpromise guarantee covers the use of ab87627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity> 95 % SDS-PAGE.
    ab87627 is purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 10mM Sodium citrate, pH 3.5

General Info

  • Alternative names
    • BMP 5
    • BMP-5
    • Bmp5
    • BMP5_HUMAN
    • Bone morphogenetic protein 5
    • MGC34244
    see all
  • FunctionInduces cartilage and bone formation.
  • Tissue specificityExpressed in the lung and liver.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant Human BMP5 protein images

  • 15% SDS-PAGE showing ab87627 at approximately 15.7kDa (3µg).
  • All lanes : Anti-BMP5 antibody (ab93852) at 1 µg/ml

    Lane 1 : Recombinant Human BMP5 protein (ab87627) at 0.1 µg
    Lane 2 : Recombinant Human BMP5 protein (ab87627) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Recombinant Human BMP5 protein (ab87627)

ab87627 has not yet been referenced specifically in any publications.

Product Wall

Application Other

Review text: I have grown human insulin-producing beta cells with or without 50ng/ml BMP5 for uptil 20 days, and followed up their ability to secrete insulin.
The presence of BMP5 did not improve the insulin secreting capacity of beta cells under these conditions.
Sample: Human Cell

Primary antibody (in addition to 'BMP5 protein (Human)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
Username

Dr. Ronit Sionov

Verified customer

Submitted Jun 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"