The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityMeasured by its ability to inhibit recombinant Human BMP2 induced alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 1-3 µg/ml in the presence of 500 ng/ml of recombinant Human BMP2.
% SDS-PAGE. ab123844 is purified by proprietary chromatographic techniques. Purity is greater than 90% as determined by HPLC and SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Store under desiccating conditions.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in sterile PBS to a concentration of 100 µg/ml. This solution can then be diluted into other aqueous buffers. Reconstituted ab123844 should be stored at 4°C for 2-7 days and at -20°C for future use. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA) and store as working aliquots at -20°C.
Activin A receptor type II like kinase 3
Activin receptor like kinase 3
Activin receptor-like kinase 3
BMP type-1A receptor
Bone morphogenetic protein receptor type IA
Bone morphogenetic protein receptor type IA precursor
Bone morphogenetic protein receptor type-1A
Serine threonine protein kinase receptor R5
Serine threonine protein kinase receptor R5 precursor
Serine/threonine-protein kinase receptor R5
FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6.
Tissue specificityHighly expressed in skeletal muscle.
Involvement in diseaseJuvenile polyposis syndrome Polyposis syndrome, mixed hereditary 2 A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 GS domain. Contains 1 protein kinase domain.