For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Our Abpromise guarantee covers the use of ab196121 in the following tested applications.
ab196121 is useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.8% Sodium chloride, 0.02% Potassium chloride, 0.05% Tween, 10% Glycerol
4-20% SDS-PAGE with Coomassie stain.
Lane 1: 4.2 µg Human BRD2 protein fragment (ab196121)
Lane 2: Protein marker
ab196121 is incubated with biotinylated acetyl-histone peptide substrate in assay buffer in a 10 µl reaction for 1 hour at RT. Proprietary Nickel Chelate acceptor beads are added, followed by proprietary Streptavidin-conjugated donor beads. After 1 hour minute incubation at RT, signal is measured.
ab196121 has not yet been referenced specifically in any publications.