Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
Lane 1: 4.5 µg Human BRD3 protein fragment (ab196122) Lane 2: Protein marker
ELISA - Human BRD3 protein fragment (ab196122)
ab196122 is incubated with biotinylated acetyl-histone peptide substrate in assay buffer in a 10 µl reaction for 1 hour at RT. Nickel Chelate acceptor beads are added, followed by Streptavidin-conjugated donor beads. After 1 hour minute incubation at RT, signal is measured.
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