The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Purified by affinity chromatography
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 50mM Potassium phosphate, 20mM Sodium chloride, pH 6.5(was adjusted by mixing of potassium dihydrogen phosphate buffer (KH2PO4) and Dipotassium hydrogen phosphate buffer (K2HPO4).
ReconstitutionReconstitute with in H2O bid
Abelson murine leukemia viral oncogene homolog 1
Abelson tyrosine protein kinase 1
ABL proto oncogene 1 non receptor tyrosine kinase
bcr/c abl oncogene protein
c abl oncogene 1 non receptor tyrosine kinase
c abl oncogene 1 receptor tyrosine kinase
Proto oncogene tyrosine protein kinase ABL1
Tyrosine-protein kinase ABL1
v abl Abelson murine leukemia viral oncogene homolog 1
FunctionProtein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
Tissue specificityWidely expressed.
Involvement in diseaseNote=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Post-translational modificationsPhosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation. Isoform IB is myristoylated on Gly-2.
Cellular localizationCytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.