Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab54318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 14mM Beta mercaptoethanol, 1mM Benzamidine, 1mM EDTA, 0.1mM PMSF, pH 7

General Info

  • Alternative names
    • Activator protein 1
    • AP 1
    • AP1
    • cJun
    • Enhancer Binding Protein AP1
    • JUN
    • Jun Activation Domain Binding Protein
    • Jun oncogene
    • JUN protein
    • Jun proto oncogene
    • JUN_HUMAN
    • JUNC
    • Oncogene JUN
    • p39
    • Proto oncogene c jun
    • Proto oncogene cJun
    • Proto-oncogene c-jun
    • Transcription Factor AP 1
    • Transcription factor AP-1
    • Transcription Factor AP1
    • V jun avian sarcoma virus 17 oncogene homolog
    • V jun sarcoma virus 17 oncogene homolog
    • V jun sarcoma virus 17 oncogene homolog (avian)
    • V-jun avian sarcoma virus 17 oncogene homolog
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog
    see all
  • Function
    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • Sequence similarities
    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE analysis of purified preparations of c-Jun exhibiting apparent MW ~ 39-kDa. The gel was stained with Coomassie Blue.

References

ab54318 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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