tRNA splicing endonuclease 15 homolog (S. cerevisiae)
tRNA splicing endonuclease subunit Sen15
tRNA-intron endonuclease SEN15
tRNA-splicing endonuclease subunit SEN15
FunctionNon-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.
Tissue specificityWidely expressed. Highly expressed in testis and uterus.
Sequence similaritiesBelongs to the SEN15 family.
Cellular localizationNucleus. Nucleus > nucleolus. May be transiently localized in the nucleolus.