Recombinant Human Calcineurin B protein (ab103786)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGNEASYPLEMCSHFDADEIKRLGKRFKKL DLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVS QFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQ QIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV
    • Molecular weight
      22 kDa including tags
    • Amino acids
      1 to 170
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab103786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab103786 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0

General Info

  • Alternative names
    • alpha isoform (calcineurin B, type I)
    • calcineurin B, type I (19kDa)
    • Calcineurin subunit B type 1
    • CALNB1
    • CANB1_HUMAN
    • Cna2
    • CNB
    • CNB1
    • OTTHUMP00000201960
    • OTTHUMP00000201961
    • Ppp3r1
    • PPP3R1 protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
    • protein phosphatase3 (formerly2B), regulatory subunit B, alpha isoform
    • Protein phosphatase 2B regulatory subunit 1
    • Protein phosphatase 2B regulatory subunit B alpha
    • protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I)
    • Protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I)
    • Protein phosphatase 3 regulatory subunit B alpha
    • Protein phosphatase 3 regulatory subunit B alpha isoform 1
    see all
  • Function
    Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
  • Sequence similarities
    Belongs to the calcineurin regulatory subunit family.
    Contains 4 EF-hand domains.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3µg ab103786.

References

ab103786 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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