Recombinant Human Calcium binding protein P22 (ab99141)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human Calcium binding protein P22 -
Purity
> 95 % SDS-PAGE.
ab99141 was purified by using conventional chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMMGSRASTLLRDEELEEIKKETGFSHSQIT RLYSRFTSLDKGENGTLSREDFQRIPELAINPLGDRIINAFFPEGEDQVN FRGFMRTLAHFRPIEDNEKSKDVNGPEPLNSRSNKLHFAFRLYDLDKDEK ISRDELLQVLRMMVGVNISDEQLGSIADRTIQEADQDGDSAISFTEFVKV LEKVDVEQKMSIRFLH -
Predicted molecular weight
25 kDa including tags -
Amino acids
1 to 195 -
Tags
His tag N-Terminus
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-
Description
Recombinant Human Calcium binding protein P22
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab99141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Calcineurin B homolog
- Calcineurin B homologous protein
- Calcineurin homologous protein
see all -
Function
Required for constitutive membrane traffic. Inhibits GTPase-stimulated Na(+)/H(+) exchange. Also inhibits calcineurin phosphatase activity. Required for activity of SLC9A1/NHE1. -
Tissue specificity
Ubiquitously expressed. Has been found in fetal eye, lung, liver, muscle, heart, kidney, thymus and spleen. -
Sequence similarities
Contains 4 EF-hand domains. -
Post-translational
modificationsBoth N-myristoylation and calcium-mediated conformational changes are essential for its function in exocytic traffic.
Phosphorylated; decreased phosphorylation is associated with an increase in exchange activity. The phosphorylation state may regulate the binding to NHE1. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99141 has not yet been referenced specifically in any publications.