Recombinant Human Caldesmon protein (ab114302)


  • Product nameRecombinant Human Caldesmon protein
  • Protein lengthFull length protein


  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionQ05682-4
    • SpeciesHuman
    • Molecular weight85 kDa including tags
    • Amino acids1 to 538


Our Abpromise guarantee covers the use of ab114302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot



  • FormLiquid
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
    This protein is best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • CAD
    • CALD 1
    • CALD1
    • Caldesmon
    • Caldesmon 1
    • Caldesmon 1 Isoform 1
    • Caldesmon 1 Isoform 2
    • Caldesmon 1 Isoform 3
    • Caldesmon 1 Isoform 4
    • Caldesmon 1 Isoform 5
    • Caldesmon1
    • CDM
    • H CAD
    • HCAD
    • L CAD
    • LCAD
    • MGC21352
    • NAG22
    see all
  • FunctionActin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
  • Tissue specificityHigh-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
  • Sequence similaritiesBelongs to the caldesmon family.
  • DomainThe N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
  • Post-translational
    In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
  • Information by UniProt

Recombinant Human Caldesmon protein images

  • 12.5% SDS-PAGE image showing ab114302 Stained with Coomassie Blue.

References for Recombinant Human Caldesmon protein (ab114302)

ab114302 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I...

Read More