Recombinant Human Calmodulin protein (ab94519)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab94519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    Purity determined to be >95% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.0

General Info

  • Alternative names
    • CALM
    • CALM 1
    • CALM 2
    • CALM 3
    • CALM_HUMAN
    • CALM1
    • CALM2
    • Calm3
    • CALML2
    • Calmodulin
    • calmodulin 1 (phosphorylase kinase, delta)
    • Calmodulin 2 (phosphorylase kinase, delta)
    • Calmodulin 3 (phosphorylase kinase, delta)
    • CaM
    • CAM I
    • CAM1
    • CAM2
    • CAM3
    • CAMB
    • CAMC
    • CAMI
    • CAMII
    • CPVT4
    • DD132
    • FLJ99410
    • LP7057 protein
    • PHKD
    • PHKD2
    • PHKD3
    • phosphorylase kinase delta
    • phosphorylase kinase, delta subunit
    see all
  • Function
    Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • Sequence similarities
    Belongs to the calmodulin family.
    Contains 4 EF-hand domains.
  • Post-translational
    modifications
    Ubiquitination results in a strongly decreased activity.
    Phosphorylation results in a decreased activity.
  • Cellular localization
    Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • Information by UniProt

Images

  • ab94519 in a sample CAMK delta kinase activation assay. The assay was performed in 25 ng CAMK delta, 200 ng/µl autocamtide-2 peptide, 0.4mM CaCl2, 50 µM ATP and trace [33P]-gamma ATP in a 25 µl reaction volume for 15 minutes at 30°C.
  • ab94519 analysed by SDS-PAGE.

References

ab94519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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The molecular weight given on website includes molecular weight of tags.
Please find attached the data regarding these two products.
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The proteins will run at ˜72kDa, ab55674 and ˜17kDa, ab94519.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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