Recombinant human CaMKI gamma protein (ab61917)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab61917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity 151 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.
    Purity > 90% as determined by SDS-PAGE and Coomassie blue staining.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Calcium/calmodulin dependent protein kinase type 1G
    • Calcium/calmodulin-dependent protein kinase I-gamma
    • Calcium/calmodulin-dependent protein kinase Ig
    • Calcium/calmodulin-dependent protein kinase type 1G
    • CaM kinase I gamma
    • CaM kinase IG
    • CaM-KI gamma
    • CaMK like CREB kinase III
    • CaMK-like CREB kinase III
    • Camk1g
    • CaMKI gamma
    • CaMKIG
    • CLICK III
    • CLICK3
    • dJ272L16.1
    • KCC1G_HUMAN
    • VWS1
    see all
  • Function
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
  • Tissue specificity
    Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • Post-translational
    modifications
    May be prenylated on Cys-473.
  • Cellular localization
    Cytoplasm. Golgi apparatus membrane. Cell membrane.
  • Information by UniProt

Images

  • ab61917 on SDS-PAGE.
  • ab61917 Kinase activity assay.

References

ab61917 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab61917.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up