Recombinant human CaMKII delta protein (ab84552)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ13557
    • SpeciesHuman
    • Molecular weight82 kDa including tags
    • Amino acids1 to 499
    • TagsGST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab84552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab84552 was determined to be 5860 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

    Other

  • FormLiquid
  • Additional notes

    ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Calcium / calmodulin dependent protein kinase 2 delta
    • Calcium / calmodulin dependent protein kinase II delta
    • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
    • calcium/calmodulin-dependent protein kinase type II delta chain
    • Calcium/calmodulin-dependent protein kinase type II subunit delta
    • CAM kinase 2 delta
    • CAM kinase II delta
    • CaM kinase II delta subunit
    • CaM kinase II subunit delta
    • CaM-kinase II delta chain
    • CAMK 2d
    • CaMK-II delta subunit
    • CaMK-II subunit delta
    • CAMK2D
    • CAMKD
    • CAMKI
    • KCC2D_HUMAN
    • RATCAMKI
    see all
  • FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
  • Tissue specificityExpressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

Recombinant human CaMKII delta protein images

  • Kinase Assay. Specific activity 5860 nmol/min/mg.
  • SDS-PAGE of ab84552. Approximate MWt 77 kDa.

References for Recombinant human CaMKII delta protein (ab84552)

ab84552 has not yet been referenced specifically in any publications.

Product Wall

The substrate used with (ab84552) Active human CaMKII delta full length protein is the synthetic peptide KKALRRQETVDAL-amide. The peptide is diluted in distilled water to 1mg/ml. Normally use around 10ul of diluted kinase for a single kinase assay.

The recombinant protein also includes an N-terminal tag which has a molecular weight of 26kDa. This is the reason we expect to see ab84552 to produce a band near 72kDa. Thanks!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"