Recombinant human CaMKII delta protein (ab84552)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CaMKII delta protein -
Biological activity
The specific activity of ab84552 was determined to be 5860 nmol/min/mg.
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Purity
> 85 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
82 kDa including tags -
Amino acids
1 to 499 -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab84552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Calcium / calmodulin dependent protein kinase 2 delta
- Calcium / calmodulin dependent protein kinase II delta
- calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
see all -
Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. -
Tissue specificity
Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. - Information by UniProt
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab84552 has been referenced in 2 publications.
- Prakash O et al. CPVT-associated calmodulin variants N53I and A102V dysregulate Ca2+ signalling via different mechanisms. J Cell Sci 135:N/A (2022). PubMed: 34888671
- Morgenstern J et al. Phosphorylation of T107 by CamKIId Regulates the Detoxification Efficiency and Proteomic Integrity of Glyoxalase 1. Cell Rep 32:108160 (2020). PubMed: 32966793