Recombinant Human Carbonic Anhydrase I protein (ab89359)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab89359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab89359 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • CA 1
    • CA I
    • CA-I
    • CA1
    • CAB
    • CAH1_HUMAN
    • CAI
    • CAN
    • Car 1
    • Car1
    • Carbonate dehydratase I
    • Carbonic anhydrase 1
    • Carbonic anhydrase A
    • Carbonic anhydrase B
    • Carbonic anhydrase B, formerly
    • Carbonic anhydrase I
    • Carbonic dehydratase
    • ECK0125
    • JW0122
    • yadF
    see all
  • Function
    Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
  • Sequence similarities
    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Recombinant Human Carbonic Anhydrase I protein images

  • 15% SDS-PAGE showing ab89359 at approximately 31kDa (3µg).
  • Anti-Carbonic Anhydrase I antibody (ab92649) at 1/500 dilution + Recombinant Human Carbonic Anhydrase I protein (ab89359) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

References for Recombinant Human Carbonic Anhydrase I protein (ab89359)

ab89359 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab89359.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up