The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin level< 0.100 Eu/µg
Additional notesThe ED50, as determined by the dose-dependent proliferation of TF-1 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg. The lyophilized protein is stable for a few weeks at room temperature, but best stored at –20°C. We recommend a quick spin followed by reconstitution in 20 mM Tris, pH 8.0 to a concentration of < 1.0mg/ml.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionThe lyophilized protein is stable for a few weeks at room temperature, but best stored at –20°C. We recommend a quick spin followed by reconstitution in 20 mM Tris, pH 8.0 to a concentration of < 1.0mg/ml.
FunctionInduces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
Tissue specificityHighly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
Sequence similaritiesBelongs to the IL-6 superfamily.