Recombinant Human CARHSP1 protein (ab116535)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9Y2V2
    • SpeciesHuman
    • SequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFATMYPYDVPDYASSEP PPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPLPTRRTRTFSATV RASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGD EVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS
    • Molecular weight44 kDa including tags
    • Amino acids2 to 147

Specifications

Our Abpromise guarantee covers the use of ab116535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

    Western blot

  • Mass spectrometry
    MALDI-TOF-TOF
  • FormLiquid
  • Additional notesab116535 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 43 % sequence coverage of CRHSP24. Theoretical pI: 6.08
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.02% Brij, 0.012% Benzamidine, 0.003% EGTA, 0.003% PMSF, 0.1% Beta mercaptoethanol, 0.79% Tris HCl, 9.24% Sucrose, 0.88% Sodium chloride

General Info

  • Alternative names
    • Calcium regulated heat stable protein (24kD)
    • Calcium regulated heat stable protein 1
    • Calcium regulated heat stable protein 1 (24kD)
    • Calcium-regulated heat stable protein 1
    • Calcium-regulated heat-stable protein of 24 kDa
    • Carhsp1
    • CHSP1_HUMAN
    • CRHSP-24
    • CRHSP24
    • CSDC1
    see all
  • FunctionBinds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).
  • Sequence similaritiesContains 1 CSD (cold-shock) domain.
  • Post-translational
    modifications
    Dephosphorylated by calcineurin in a Ca(2+) dependent manner.
  • Cellular localizationCytoplasm. Cytoplasm > P-body. Cytoplasmic granule. Detected at cytoplasmic stress granules and P-bodies. Detected at exosome granules where mRNA is degraded.
  • Information by UniProt

Recombinant Human CARHSP1 protein images

  • 12 % SDS-PAGE gel showing ab116535.

References for Recombinant Human CARHSP1 protein (ab116535)

ab116535 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. I will be helping you while Carolyn is out of the office.

I would imagine that you could run non-reducing conditions, though we haven't specifically tested this. If you're going to be detecing the protein with an ant...

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Application Western blot

Review text: We used this HA-tagged CARHSP1 protein only as a control to quantify our own HA-tagged proteins on a Western blot. For this it worked well, although there are weak degradation products as well as the main 44 kDa band for CARHSP1.
Sample: Human Recombinant protein
Loading amount: 0.3 µg
Treatment: diluted in 0.8M Tris, 0.2M sorbitol, 1% betamercaptoethanol, with bromophenol blue. Added 1% SDS then boiled for 5 min.
Gel Running Conditions: Reduced Denaturing (15% acrylamide gel)
Blocking step: Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 10°C

Other product details
Dilution: 1/100
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS + 0.1% tween

Primary antibody (in addition to 'CARHSP1 protein')
Name: Non-Abcam antibody was used: anti-HA
Host species: Rabbit
Dilution: 1/2000

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit ECL
Clonality: Polyclonal
Dilution: 1/5000

Detection
Detection method: ECL
Exposure: 4 second(s)
Bands: Specific: 44 kDa Non-specific: 30, 35 kDa

Additional data
Additional Notes: Non-specific bands (30, 35kDa) may be due to degradation.
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Abcam user community

Verified customer

Submitted Sep 24 2012

Thank you for your email and your patience.

The producers of this protein confirmed that these bands are degradation products. Unfortunately we do not have another batch without these bands.I am sorry for this inconvenience.

Please ...

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I am very pleased to hear you would like to accept our offer and test ab116535 in Western blot. This code will give you: 1 free protein or value off another product before the expiration date. To redeem this offer, please submit an Abreview ...

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Thank you very much for your interest in ab116535.

Unfortunately we do not have aliquots of our products in stock which we can send out for testing purposes. However I would be pleased to make you the following offer: We do not currently hav...

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Thank you for your patience.

We haveone protein in our catalog currently which has a HA tag and could be useful for you. It is the ab116535, a tagged CARHSP1 protein which runs at 44kD in Western blot. http://www.abcam.com/index.html?datashee...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"