Recombinant human Caspase-4 protein (Active) (ab51994)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies
Description
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Product name
Recombinant human Caspase-4 protein (Active) -
Biological activity
One unit of the recombinant caspase-4 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol and 10 mM DTT.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab51994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
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Form
Lyophilized -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 1084 Caspase-4, human recombinant. 1084-100 is the same size as the 100 unit size of ab51994.
This protein is a heterotetramer consisting of two large and two small subunits. Active Caspase 4 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays.
5000 units/mg.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 15% Glycerol (glycerin, glycerine), PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute to 1 unit per µl in PBS containing 15% glycerol. Aliquot and store immediately at -80°C.
General Info
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Alternative names
- Apoptotic cysteine protease Mih1/TX
- CASP-4
- CASP4
see all -
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. -
Tissue specificity
Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab51994 has been referenced in 3 publications.
- Amara N et al. Selective activation of PFKL suppresses the phagocytic oxidative burst. Cell 184:4480-4494.e15 (2021). PubMed: 34320407
- Chen Q et al. Nitrosonisoldipine is a selective inhibitor of inflammatory caspases and protects against pyroptosis and related septic shock. Eur J Immunol 51:1234-1245 (2021). PubMed: 33454984
- Sollberger G et al. Gasdermin D plays a vital role in the generation of neutrophil extracellular traps. Sci Immunol 3:N/A (2018). PubMed: 30143555