Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP55211
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab52203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySPECIFIC ACTIVITY: 400 units/mg
  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.

  • FormLyophilised
  • Additional notesUNIT DEFINITION: One unit of the recombinant Caspase 9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 15% Glycerol, PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute to 1 unit per µl in PBS containing 15% glycerol. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C.

General Info

  • Alternative names
    • APAF-3
    • APAF3
    • Apoptosis related cysteine peptidase
    • Apoptotic protease Mch-6
    • Apoptotic protease-activating factor 3
    • CASP-9
    • CASP9
    • CASP9_HUMAN
    • Caspase 9 apoptosis related cysteine peptidase
    • Caspase 9 Dominant Negative
    • Caspase 9c
    • Caspase-9
    • Caspase-9 subunit p10
    • ICE LAP6
    • ICE like apoptotic protease 6
    • ICE-LAP6
    • ICE-like apoptotic protease 6
    • MCH6
    • PPP1R56
    • protein phosphatase 1, regulatory subunit 56
    • RNCASP9
    see all
  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt

Recombinant human Caspase-9 protein images

  • Lane 1: Recombinant caspase 9 protein (Active) ab52203- 100 ng. Lane 2: BSA- 400 ng. Lane 3: BSA- 200 ng. Lane 4: BSA- 100 ng. Lane 5: BSA- 50 ng. Lane 6: BSA- 25 ng. SYPRO Ruby protein stain. Predicted band size: 46 kDa (precursor protein) and 35 kDa (p35 subunit) Observed Bands: 20 kDa and 11 kDa.

References for Recombinant human Caspase-9 protein (ab52203)

This product has been referenced in:
  • Basu A  et al. Proteolytic activation of protein kinase C-epsilon by caspase-mediated processing and transduction of antiapoptotic signals. J Biol Chem 277:41850-6 (2002). Read more (PubMed: 12198125) »
  • Azuma T  et al. Gelsolin in complex with phosphatidylinositol 4,5-bisphosphate inhibits caspase-3 and -9 to retard apoptotic progression. J Biol Chem 275:3761-6 (2000). Read more (PubMed: 10660524) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

I have spoken to tha lab regarding ab52203 and please find their response below:

This protein is N-terminal His Tagged.
The mol wt of this product is ˜30 kDa.
The amount of protein in terms of &...

Read More

Thank you for contacting Abcam.

From the attached data it appears that the antibody 1 and 2 recognize the band which is ˜44 kDa. In addition to the 45 kDa procaspase-9 and 35/37 kDa cleaved large fragment there is also a 25 kDa fully cleaved ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"