The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab117172 is purified by proprietary chromatographic techniques.
Additional notesStore at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Please see notes section.
pH: 8.50 Constituents: 0.32% Tris HCl, 10% Glycerol
15 hydroxyprostaglandin dehydrogenase [NADP+]
15-hydroxyprostaglandin dehydrogenase [NADP+]
Carbonyl reductase [NADPH] 1
Carbonyl Reductase 1
NADPH dependent carbonyl reductase 1
NADPH-dependent carbonyl reductase 1
Prostaglandin 9 ketoreductase
Prostaglandin E(2) 9 reductase
FunctionNADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.