Recombinant Human CBR1 protein (ab85336)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMSSGIHVALV TGGNKGIGLA IVRDLCRLFS GDVVLTARDV TRGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLDVLVNN AGIAFKVADP TPFHIQAEVT MKTNFFGTRD VCTELLPLIK PQGRVVNVSS IMSVRALKSC SPELQQKFRS ETITEEELVG LMNKFVEDTK KGVHQKEGWP SSAYGVTKIG VTVLSRIHAR KLSEQRKGDK ILLNACCPGW VRTDMAGPKA TKSPEEGAET PVYLALLPPD AEGPHGQFVS EKRVEQW

Specifications

Our Abpromise guarantee covers the use of ab85336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab85336 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.5

General Info

  • Alternative names
    • 15 hydroxyprostaglandin dehydrogenase [NADP+]
    • 15-hydroxyprostaglandin dehydrogenase [NADP+]
    • Carbonyl reductase [NADPH] 1
    • Carbonyl Reductase 1
    • CBR 1
    • CBR1
    • CBR1_HUMAN
    • CRN
    • NADPH dependent carbonyl reductase 1
    • NADPH-dependent carbonyl reductase 1
    • Prostaglandin 9 ketoreductase
    • Prostaglandin 9-ketoreductase
    • Prostaglandin E(2) 9 reductase
    • Prostaglandin-E(2) 9-reductase
    • SDR21C1
    see all
  • FunctionNADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant Human CBR1 protein images

  • 15% SDS-PAGE showing ab85336 at approximately 30kDa (3µg).

References for Recombinant Human CBR1 protein (ab85336)

ab85336 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot

Review text: We have used Abcam's CBR1 recombinant protein on a number of projects to confirm the activity of CBR1 primary antibodies and the specificity of CBR3 (a closely related enzyme) antibodies. Additionally, we have successfully used this human protein to create standard curves (image included) for quantitative analysis of protein amounts in human liver and heart tissue lysates.
Sample: Human Recombinant protein (Recombinant protein)
Loading amount: 0.3 µg
Specification: Recombinant protein
Blocking step: Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details
Incubation time: 1 hour(s) and 10 minute(s) · Temperature: 25°C · Diluent: PBS-tween (0.05%)

Primary antibody (in addition to 'CBR1 protein (Human)')
Name: Non-Abcam antibody was used: Carbonyl reductase 1 (C-18) Antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/2500

Secondary antibody
Name: Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection
Detection method: ECL
Exposure: 30 second(s)
Bands: Specific: 32 kDa
Positive control: No positive control was necessary given that the recombinant CBR1 protein comprised our protein of interest.

Additional data
Additional Notes: PVDF membranes were used in our WB. The image of the standard curve shows the CBR1 recombinant protein in increasing amounts from left to right (0.05, 0.1, 0.15, and 0.30 ug) and a protein ladder on the far right as detected using the conditions outlined above.
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Submitted Jan 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"