Recombinant Human CBR3 protein (ab86848)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET PVYLALLPPD ATEPQGQLVH DKVVQNW

Specifications

Our Abpromise guarantee covers the use of ab86848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab86848 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0

General Info

  • Alternative names
    • Carbonyl reductase (NADPH) 3
    • Carbonyl reductase (NADPH) 3 EC 1.1.1.184
    • Carbonyl reductase [NADPH] 3
    • Carbonyl reductase 3
    • CBR 3
    • CBR3
    • CBR3_HUMAN
    • EC 1.1.1.184
    • Epididymis secretory protein Li 25
    • hCBR3
    • HEL S 25
    • NADPH dependent carbonyl reductase 3
    • NADPH-dependent carbonyl reductase 3
    • SDR21C2
    • Short chain dehydrogenase/reductase family 21C member 2
    see all
  • Function
    Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).
  • Tissue specificity
    Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Recombinant Human CBR3 protein images

  • 15% SDS-PAGE showing ab86848 at approximately 33kDa (3µg).

References for Recombinant Human CBR3 protein (ab86848)

ab86848 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot

Review text: We recently started using Abcam's CBR3 recombinant protein to confirm the activity of CBR3 primary antibodies. Additionally, we have successfully used this human protein to create standard curves (image included) for quantitative analysis of protein amounts in human liver tissue lysates.
Sample: Human Recombinant protein (Recombinant protein)
Loading amount: 0.03 µg
Specification: Recombinant protein
Gel Running Conditions: Non-reduced Denaturing (12% Gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Dilution: 1/30
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-tween (0.05%)

Primary antibody (in addition to 'CBR3 protein (His tag)')
Name: Non-Abcam antibody was used: Carbonyl reductase 3 (C-16)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/1000

Secondary antibody
Name: Non-Abcam antibody was used: Anti-Goat IgG (whole molecule)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000

Detection
Detection method: ECL
Exposure: 1 minute(s) and 0 second(s)
Bands: Specific: 30 kDa
Positive control: No positive control was necessary given that the recombinant CBR3 protein comprised our protein of interest.

Additional data
Additional Notes: Nitrocellulose membranes were used in our WB. The image of the standard curve shows the CBR3 recombinant protein in increasing amounts from left to right (0.03, 0.07, and 0.10 ug) and a protein ladder on the far right as detected using the conditions outlined above.
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Submitted Nov 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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