Recombinant Human CBR4 protein (ab101110)

Overview

  • Product nameRecombinant Human CBR4 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ8N4T8
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMDKVCAVFGGSRGIGRAVAQLMARKGYRLA VIARNLEGAKAAAGDLGGDHLAFSCDVAKEHDVQNTFEEMEKHLGRVNFL VNAAGINRDGLLVRTKTEDMVSQLHTNLLGSMLTCKAAMRTMIQQQGGSI VNVGSIVGLKGNSGQSVYSASKGGLVGFSRALAKEVARKKIRVNVVAPGF VHTDMTKDLKEEHLKKNIPLGRFGETIEVAHAVVFLLESPYITGHVLVVD GGLQLIL
    • Molecular weight28 kDa including tags
    • Amino acids1 to 237
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab101110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    ab101110 was purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 5mM DTT, 200mM Sodium chloride, 20mM Tris HCl, pH 8.0

General Info

  • Alternative names
    • 3 oxoacyl [acyl carrier protein] reductase
    • 3-oxoacyl-[acyl-carrier-protein] reductase
    • Carbonic reductase 4
    • Carbonyl reductase 4
    • Carbonyl reductase family member 4
    • CBR 4
    • Cbr4
    • CBR4_HUMAN
    • FLJ14431
    • Quinone reductase CBR4
    • SDR45C1
    • Short chain dehydrogenase/reductase family 45C member 1
    see all
  • FunctionThe heteroteramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria. The homotetramer may act as NADPH-dependent quinone reductase. Has broad substrate specificity and reduces 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro).
  • Tissue specificityDetected in liver and kidney (at protein level).
  • PathwayLipid metabolism; fatty acid biosynthesis.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt

Recombinant Human CBR4 protein images

  • 15% SDS-PAGE analysis of 3µg ab101110.

References for Recombinant Human CBR4 protein (ab101110)

ab101110 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot

Review text: This CBR4 recombinant protein worked beautifully for our Western blotting positive control purposes.
Sample: Human Recombinant protein (Human rCBR4)
Loading amount: 0.03 µg
Specification: Human rCBR4
Gel Running Conditions: Non-reduced Denaturing (12% Tris/glycine gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Concentration: 1e-005 µg/ml
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-Tween 20 (0.05%)

Primary antibody (in addition to 'CBR4 protein')
Primary antibody: Abcam primary antibody: Anti-CBR4 antibody (ab115860)
Dilution: 1/1000

Secondary antibody
Name: Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000

Detection
Detection method: ECL
Exposure: 1 minute(s) and 0 second(s)
Bands: Specific: 32 kDa
Positive control: We used this product as a positive control for western blotting of human CBR4.

Additional data
Additional Notes: Nitrocellulose membranes were used in our WB. The image shows the CBR4 recombinant protein in increasing amounts from left to right and a protein molecular weight ladder on the far right with corresponding molecular weights labeled.
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Submitted Nov 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"